Low Dose Insemination Techniques


  • To obtain acceptable pregnancy rates, the minimum recommended number of spermatozoa is:
      • 300 million progressively motile spermatozoa for fresh semen
    • 200 million progressively motile spermatozoa for frozen semen
    • 500 million progressively motile spermatozoa for shipped cooled semen
  • Insemination with less than 100 million progressively forward spermatozoa results in unsatisfactory pregnancy rates.
  • Interest in low dose insemination techniques results from:
    • The advent of fluorescence activated cell separation technology that can be used to sort X and Y-chromosomes. Once sorted, low numbers of spermatozoa are available for insemination and the sex of the foal can be chosen at the time of breeding.
    • The use of epididymal spermatozoa harvested after death or from stallions with occluded ducts.
    • Improve fertility of sub fertile stallions and mares.
    • Lower doses of frozen semen.


  • Rectally guided deep uterine insemination
  • Hysteroscopic, using endoscope to visualize the opening of the oviduct in the uterus and target it for deposition of semen.

Pregnancies can be consistently obtained using as little as 1 million spermatazoa

Higher pregnancy rates resulted when using hysteroscopic insemination over conventional insemination using less than 3 million motile sperm from frozen thawed semen.

Pregnancy rates are similar between deep uterine and hysteroscopic insemination techniques when using 5 million motile sperm per dose.

  • Hysteroscopic insemination is the most useful when using very small doses, such as sexed semen or when very poor motility exists.
Don’t Skip the Purchase Exam
Intracytoplasmic Sperm Injection (ICSI)

Latest News

Reata Equine Veterinary

Reata Equine Veterinary Group is a full-service mobile equine-only veterinary practice in Southern Arizona dedicated to providing the best medical and wellness care for your horse.

Horse ownership is a joy

24/7 Emergency Services

If you call the office with an emergency during regular hours our friendly staff will listen to your emergency and provide immediate help. If the emergency happens after hours, call (520) 749-1446 and the answering machine will connect you to the veterinarian on call so that you receive the help you need.

[email protected]

We Accept