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Low Dose Insemination Techniques

INTRODUCTION

  • To obtain acceptable pregnancy rates, the minimum recommended number of spermatozoa is:
      • 300 million progressively motile spermatozoa for fresh semen
    • 200 million progressively motile spermatozoa for frozen semen
    • 500 million progressively motile spermatozoa for shipped cooled semen
  • Insemination with less than 100 million progressively forward spermatozoa results in unsatisfactory pregnancy rates.
  • Interest in low dose insemination techniques results from:
    • The advent of fluorescence activated cell separation technology that can be used to sort X and Y-chromosomes. Once sorted, low numbers of spermatozoa are available for insemination and the sex of the foal can be chosen at the time of breeding.
    • The use of epididymal spermatozoa harvested after death or from stallions with occluded ducts.
    • Improve fertility of sub fertile stallions and mares.
    • Lower doses of frozen semen.

METHODS

  • Rectally guided deep uterine insemination
  • Hysteroscopic, using endoscope to visualize the opening of the oviduct in the uterus and target it for deposition of semen.

RESULTS
Pregnancies can be consistently obtained using as little as 1 million spermatazoa

Higher pregnancy rates resulted when using hysteroscopic insemination over conventional insemination using less than 3 million motile sperm from frozen thawed semen.

Pregnancy rates are similar between deep uterine and hysteroscopic insemination techniques when using 5 million motile sperm per dose.

  • Hysteroscopic insemination is the most useful when using very small doses, such as sexed semen or when very poor motility exists.
Don’t Skip the Purchase Exam
Intracytoplasmic Sperm Injection (ICSI)

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